浅谈动科专业课程多媒体教学中存在的问题与对策

多媒体技术作为一种全新的教学手段走进课堂。本文阐述了动科专业课程多媒体教学的优越性,分析了存在的问题,同时提出一些建议,以期望能充分发挥多媒体技术的优点,更好的提高动科专业课程的教学效果。     

旋转法处理平面度误差测量数据的计算机辅助分析

平面度误差测量中常采用旋转法处理数据,使之符合最小条件,从而计算出平面度误差。但手工处理过程复杂,工作量大。使用我们自主开发的计算机辅助分析软件,不仅可以提高运算速度,而且可以通过CAD图形分析模块的分析,为生产加工提出指导性意见。     

鳜胃蛋白酶原基因cDNA全长的克隆与序列分析

利用RT–PCR和cDNA末端快速扩增法(RACE)克隆鳜(Siniperca chuatsi)胃蛋白酶原基因cDNA全长序列，并对该基因的结构特征和系统进化关系进行了分析。鳜胃蛋白酶原基因cDNA序列全长1367 bp，5′端非翻译区43 bp，3′端非翻译区187 bp，开放阅读框(ORF)1137 bp，共编码378个氨基酸。鳜胃蛋白酶原氨基末端存在信号肽和激活肽序列，序列中含有催化活性必需的2个天冬氨酸残基和构成二硫键的6个半胱氨酸残基。鳜胃蛋白酶原氨基酸序列与其他脊椎动物胃蛋白酶原氨基酸序列的同源性为59.9%～91.2%，表明胃蛋白酶原基因在脊椎动物的长期进化中比较保守。鳜胃蛋白酶原基因的成功克隆不仅为进一步研究该基因的时空表达奠定基础，而且为鱼类胃蛋白酶原的分子特征和进化提供了新的资料。     

Isolation and characterization of microsatellites in Bambusa arundinacea and cross species amplification in other bamboos

Isolation and characterization of microsatellites was analysed in Bambusa arundinacea and cross species amplification studied in other bamboos. Microsatellites, tandem repeats of short nucleotide (1–6 bp) sequences, are the DNA marker of choice because of their highly polymorphic, ubiquitous distribution within the genome, ease of genotyping through Polymerase chain reaction, selectively neutral, co‐dominant and multiallelic nature. Six microsatellites, three polymorphic and three monomorphic have been characterized for the first time in a bamboo species, Bambusa arundinacea belonging to the family Poaceae. The numbers of alleles per locus ranged from 2 to 13. Cross species amplification was tested in 18 other bamboo species. Monomorphic simple sequence repeats (SSRs) were found to be cross amplified in most of the species tested and polymorphic ones in only three to four species. The utility of SSR loci in a genetic diversity study of B. arundinacea and other cross‐amplified bamboo species has been discussed. This study will help in population genetic studies in bamboo species.     

COMPUTER ALDED DESIGN OF FORMING CUTTER FOR REVISING INVOLUTE HELICAL

In this paper, a calculation method of forming cutter contour of involute helical with arbitrary easement is presented, the segmantal arcs of the best uniform approximation and tolerance to fit smoothly the cutter contour points are used the profile on the cross section of the helical is calculated and the design of the cutter is examined. The method can be used to design precision forming cutter of involute helical with the arbitrary easement. It is valuable in engineering application.     

COMPUTER AIDED DESIGN OF THE CORE AND CAVITY OF PLASTIC INJECTION MOULD

This paper discusses the designing of the injection mould for producing the plastic case of TV set carried out in the type IBM PC/XT (or Great Wall 0520)micro-eomputer. The procedure consists of inputting the 2-D drawings of the TV set case with the aid of sub-drawing, coding and menu specifying; setting up the mathematic model according to the relationship between the plastic part and the mould; designing the core and caving of the mould and finally displaying and plotting the drawing of the mould. The connection between the Auto CAD software and the advanced language for orogramming and the data convertion is also discussed.     

Evaluation of 'quick and dirty' DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.)

Six simple methods for extracting DNA from rice seedlings were evaluated for marker‐assisted selection (MAS). The assessment of each method was based on PCR amplification of SSR markers, DNA yield and purity, time and cost. Based on these criteria, two methods were selected as being superior to other methods. The best two methods included the standard method developed at the International Rice Research Institute (IRRI), which utilizes a sodium dodecyl sulfate extraction buffer followed by chloroform/isoamyl alcohol extraction and a previously published method using sodium hydroxide and Tris. These two methods produced nearly identical PCR amplification results. The sodium hydroxide method is considerably simpler, quicker and cheaper than the standard IRRI method, and may be particularly useful for many applications of MAS or high‐resolution mapping. This method was also adapted into an effective high throughput method utilizing 96‐well plates emphasizing its versatility.     

小型拖拉机发动机功率油耗计算机辅助测试系统的研制

在吸收机械式功率、油耗测试仪的基础上，利用机电一体化等先进技术，研制成一套小型拖拉机发动机功率、油耗计算机辅助测试系统。实现了对功率、油耗、转速的计算机辅助测试及曲线绘制。该系统采用了动态实时图像显示技术及在线数字屏幕输出，提高了系统的实用性。